Sometimes, experiments are too big, too expensive, or too specialist to do yourself or to negotiate a collaboration with someone who can. Fortunately there is a way for some of you to get those experiments done – but as ever, it involves competing for funding. Today I’ll highlight two service providers who are taking applications from researchers for a limited number of fully funded services.
The BBSRC are funding the Community Resource for Wheat Transformation at NIAB. NIAB scientists are wheat transformation specialists, and use a non-commercialized method with over 30% success rate – higher than standard Agrobacterium-mediated wheat transformation (Harwood, 2012). The ‘Community Resource’ is 50 single gene transformations, which researchers must apply for. Half of the transformations are reserved for model plant researchers wanting to test a gene of interest in wheat. The application form is fairly straightforward, requesting information about the proposed gene and research; and how it links to BBSRC food security targets. You need to apply by Thursday this week. If successful, the researcher provides NIAB with a gene in an Entry construct flanked by aatL sequences. NIAB performs the transformation, and delivers the researcher 30 inpendent transformed plants as either plantlets or T1 seed, having confirmed transgenesis by PCR or QPCR.
The Centre for Plant Integrative Biology at the University of Nottingham and the National Plant Phenomics Centre at Aberystwyth University is just one of 14 participants in the European Plant Phenotyping Network (EPPN; Ruth blogged about the centre in Julich a few weeks ago). Researchers can apply for access to CPIB (or another European EPPN installation) to do a phenotyping experiment. ‘Access’ includes:
- free access for eligible user groups to research facilities;
- support for travel;
- on-site logistic support by the infrastructure staff;
- access to knowledge and know-how at the research infrastructures necessary to complete the proposed experimental work