GARNet Research Roundup: October 10th 2018

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Published on: October 10, 2018

This edition of the GARNet research roundup begins with a paper from Jose Gutierrez-Marcos’ lab in Warwick that investigates the functional significance of inherited epigenetics marks in clonally propagated plants. Second is work from Sara Simonini and Lars Ostergaard (John Innes Centre) that defines a domain in the ETTIN protein important for the auxin response. Next is work from SLCU from Siobhan Braybrook and Henrik Jonsson that experimentally defines and models the role of cell wall composition in anisotropic hypocotyl growth. The fourth paper is from Jonathan Jones’ lab (TSL, Norwich) that adds to our understanding of the activity of the RRS1-R-RPS4 NLR immune complex.

The final three papers are each from the University of Edinburgh and look at different aspects of the relationship between light quality and the circadian clock. First is a paper from Karen Halliday’s lab that investigates the role of PHYA; next Andrew Millar is a co-author on a manuscript that looks at control of FT expression during seasonally realistic conditions. Finally Ference Nagy and Mirela Domijan (University of Liverpool) co-author a paper that assesses the role of HY5 in the response to blue-light.

Wibowo A, Becker C, Durr J, Price J, Spaepen S, Hilton S, Putra H, Papareddy R, Saintain Q, Harvey S, Bending GD, Schulze-Lefert P, Weigel D, Gutierrez-Marcos J (2018) Partial maintenance of organ-specific epigenetic marks during plant asexual reproduction leads to heritable phenotypic variation. Proc Natl Acad Sci U S A doi: 10.1073/pnas.1805371115

Open Access
Anjar Wibowo and Claude Becker are first authors on this UK-German collaboration from the labs of Jose Gutierrez-Marcos (University of Warwick) and Detlef Weigel (Max Planck Institutem, Tübingen). In this work they clonally propagate Arabidopsis and show that organ-specific epigenetic marks are maintained across generations. Interestingly these changes are then maintained through multiple rounds of sexual reproduction. These epigenetic marks provide heritable molecular and physiological phenotypes that can alter the response to pathogens, allowing progeny to maintain a beneficial epigenome that was generated in their parents.

Simonini S, Mas PJ, Mas CMVS, Østergaard L, Hart DJ (2018) Auxin sensing is a property of an unstructured domain in the Auxin Response Factor ETTIN of Arabidopsis thaliana. Sci Rep. doi: 10.1038/s41598-018-31634-9

Open Access

This UK-France collaboration is led by Sara Simonini from the John Innes Centre and continues the Ostergaard lab’s work on the role of the auxin response factor ETTIN in the auxin response. In this paper they analyse the C-terminal ETT specific domain (ES domain) across plant lineages, showing that it does not directly bind auxin but could functional response to a dose response of auxin in a Y2H assay. Understanding more about this ES domain will increase our understanding of auxin sensing by ETTIN and more broadly about auxin-dependent gene regulation.

Bou Daher F, Chen Y, Bozorg B, Clough J, Jönsson H, Braybrook SA. Anisotropic growth is achieved through the additive mechanical effect of material anisotropy and elastic asymmetry. Elife.  doi: 10.7554/eLife.38161

Open Access

Firas Bou Daher is the first author on work from Siobhan Braybrook’s lab conducted both in the Sainsbury Lab Cambridge University and at its new home in California. In this work they look at anisotropic growth in the Arabidopsis hypocotyl and the relationship between cellulose orientation and pectin deposition in the control of this process. They provide experimental evidence that growth parameters are influenced by pectin biochemistry in processes that begin immediately after germination.

Ma Y, Guo H, Hu L, Martinez PP, Moschou PN, Cevik V, Ding P, Duxbury Z, Sarris PF, Jones JDG (2018) Distinct modes of derepression of an Arabidopsis immune receptor complex by two different bacterial effectors. Proc Natl Acad Sci U S A. doi: 10.1073/pnas.1811858115

Yan Ma and Hailong Guo are lead authors on this study from Jonathan Jones’ lab at The Sainsbury Lab, Norwich. They perform a detailed examination of the RRS1-R-RPS4 NLR protein complex, which is necessary to respond to at the bacterial effectors, AvrRps4 and PopP2. Deletion of a WRKY transcription factor domain in the RRS1-R protein causes constitutive activation of the defense response, indicating that this domain maintains the complex in an inactive state in the absence of pathogens. Indeed AvrRps4 does interact with this WRKY domain but interestingly PopP2 activation requires interaction with a longer C-terminal extension of RRS1-R. This demonstrates that although these bacterial effectors are recognised by the same complex the interactions occurs in a subtly but functionally distinct ways.

Seaton DD, Toledo-Ortiz G, Ganpudi A, Kubota A, Imaizumi T, Halliday KJ (2018) Dawn and photoperiod sensing by phytochrome A. Proc Natl Acad Sci U S A. doi: 10.1073/pnas.1803398115

Open Access

This research from Karen Halliday’s lab in Edinburgh is led by Daniel Seaton and provided a detailed assessment of the role of phytochrome A (phyA) in photoperiod sensing, which is defined as the relationship between the circadian clock and external light signals. They show that PHYA activity, controlled by the transcription factors, PIF4 and PIF5, is a key regulator of morning activity, particularly in short photoperiods. PHYA protein accumulates during the night and responds to light by promoting a burst of gene expression that prepares the plant for the upcoming daylight and places this light receptor as a key detector of dawn.

Song YH, Kubota A, Kwon MS, Covington MF, Lee N, Taagen ER, Laboy Cintrón D, Hwang DY, Akiyama R, Hodge SK, Huang H, Nguyen NH, Nusinow DA, Millar AJ, Shimizu KK, Imaizumi T (2018) Molecular basis of flowering under natural long-day conditions in Arabidopsis. Nat Plants. doi: 10.1038/s41477-018-0253-3

Andrew Millar is a co-author on this US-led paper that investigates the circadian regulation of the Arabidopsis florigen gene FLOWERING LOCUS T (FT) within an annual context, showing that during the spring FT shows a morning peak is absent in their usual lab experiments. By adjusting growth-room conditions to mimic natural seasonal variations they show that phytochrome A and EARLY FLOWERING 3 regulate morning FT expression by stabilizing the CONSTANS protein. This manuscript highlights the importance of providing seasonal-specific conditions in order to understand field-relevant regulation of plant growth.

Hajdu A, Dobos O, Domijan M, Bálint B, Nagy I, Nagy F, Kozma-Bognár L. ELONGATED HYPOCOTYL 5 mediates blue light signalling to the Arabidopsis circadian clock (2018) Plant J. doi: 10.1111/tpj.14106

Ferenc Nagy (University of Edinburgh) is a co-author on this Hungarian-led study that looks the effect of light quality on the function of the key signaling hub transcription factor ELONGATED HYPOCOTYL 5 (HY5). They show that hy5 mutants show shorter period rhythms in blue but not in red light or darkness. Even though the pattern and level of HY5 alters its binding to downstream promotor elements, subsequent gene expression is only altered in a few genes. In collaboration with Mirela Domijan (University of Liverpool) they model this response to suggest that clock feedback mechanisms mask HY5-induced changes. Ultimately they show that HY5 is important in decoding the blue:red mix of white light and that it at least partially informs activity of the circadian oscillator.

High Value Chemicals from Plants Annual Meeting 2018

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Published on: October 3, 2018
Naomi Nakayama discusses plant cell factories

The final annual meeting in Phase I of the BBSRC-funded HVCfP NIBB network was held on October 1st in the delightful Royal College of Physicians, close to Regents Park in London. This single day meeting was a byte-size mix of invited talks and those provided by researchers who had received Proof of Concept (PoC) or Business Interaction Voucher (BIV) funding from the network.

From a GARNet perspective it was gratifying to hear presentations that included preliminary work conducted in Arabidopsis, demonstrating the importance of model organisms in the development of ideas that can lead to industrial biotechnology projects. Naomi Nakayama from the University of Edinburgh described her labs work aimed at optimising use of Arabidopsis cell cultures as well as in developing plant stem cells as ‘single cell factories’. Secondly Peter Eastmond from Rothamsted Research described the initial characterisation of the Sugar Dependent1 hydrolase enzyme that they are now developing as a potential industrial biocatalyst.

Paul Fraser from RHUL and Mike Roberts from Lancaster University introduced very different research projects that both use tomato plants. Long-term establishment of RIL lines have allowed the Fraser lab to identify tomato plants with increased levels of B-carotene in the fruit. This project has similarity to other attempts at vitamin A biofortification yet takes advantage of many years expertise working specifically with this plant. These B-carotene fortified lines are now ready for the field and should be particularly important in regions with high food insecurity and vitamin A deficiency.

Mike Roberts has a nascent industrial collaboration with greenhouse tomato producer APS Salads. Their soil-free growth of tomatoes generates a large amount of waste biomass, which is currently used for a variety of applications that rely on downstream anaerobic decomposition. It is known that mechanical disruption of plant tissue causes the release of protective defence chemicals so the Roberts lab have used HVCfP BIV funding to investigate whether macerated tomato waste has protective anti-pathogen properties. The initial characterisation of liquid fractions taken from the waste pipeline have given promising protective effects indicating that the mechanical disruption of the tissue generates an as-yet-unknown defense-promoting compound.

Michael Marsden discusses co-products, not plant waste.

On a related note, Michael Marsden provided an invited talk and asked delegates to re-think the idea of ‘plant waste’. His company AB Connect labels waste as ‘co-products’ and it was extremely informative to learn about all the possible uses of crop co-products across a range of industries. However there certainly remains additional potential in this area as technologies continue to develop for degradation of cellulosic material and improvement of manufacturing pipelines.

Sweet smelling success story of Oxford BioTrans.

Jason King from Oxford Biotrans provided the opening invited talk that was a real success story of activities that have taken place since he last presented at the 2015 annual HVCfP meeting. Their main product is the grapefruit flavour nootkatone that they produce from oranges using patented P450 enzymes. This industrial project was recently highlighted as a success story by the BBSRC. Oxford BioTrans are now investigating options for producing a range of other products using their set of novel P450s. Pleasingly Jason King reported that they have not had significant difficulties in obtaining funding for this project both from national funding bodies and local angel investors.

The afternoon invited speakers provided a different perspective on some wider issues surrounding the research environment. Kelly Vere is working with the Science Council on the establishment of the Technicians Committment, which is an initiative to provide recognition for the vital yet often underappreciated support provided by technical staff in higher education. Over 50 universities have signed up to the charter and many are taking steps to provide this extra support.

Alison Prendiville (University of the Arts London) and Sebastian Fuller (St George’s, University of London) described their involvement with the EU-funded Pharma-Factory project. This involves the input of numerous stakeholders associated with the use of the products generated by plant-based biofactories. These include potential patients, clinicians, regulators and researchers. They described how they are using the process of co-design to create partnerships that take into account stakeholder priorities in order to facilitate new methods of knowledge exchange. Intuitively it seems that this type of project might be challenging for bench scientists to fully appreciate so it will be interesting to observe where this project leads and to learn about their conclusions.

Due to the obvious links between the GARNet community and the type of PoC/BIV projects funded by the HVCfP network, the GARNet coordinator has attended and participated in a number of HVCfP events over the past four years. Although this annual meeting only highlighted a small set of supported projects it seems clear that the HVCfP network has succeeded in bringing together academics and industrial partners as well as supporting research in its early stages.

The decision regarding Phase II of the NIBBs will be announced over the next month so hopefully this plant-based network will gain follow-on funding to continue the progress they have made during Phase I.

Andrew Millar talks Open Data at GARNet2018

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Published on: October 2, 2018

Andrew Millar (University of Edinburgh) speaks at the GARNet2018 conference about ‘Being more Open by being more Productive’

GARNet Arabidopsis Roundup: September 13th

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Published on: September 13, 2018

The latest GARNet Research Roundup includes two papers led by Cyril Zipfel from The Sainsbury lab, Norwich (who has since moved his lab to the University of Zurich). The first paper demonstrates the potential for cross-species strategies for developing disease resistance whilst the second is a phosphoproteomic dissection of the BAK1 immune co-receptor. Third is work from the lab of Liam Dolan (University of Oxford) that has discovered a novel type of regulation for RSL Class I bHLH transcription factors in Marchantia. Finally is research from Paul Dupree’s lab in Cambridge that investigates the structure of galactoglucomannan polysaccharides in the Arabidopsis cell wall.

Pfeilmeier S, George J, Morel A, Roy S, Smoker M, Stransfeld L, Downie JA, Peeters N, Malone JG, Zipfel C (2018) Expression of the Arabidopsis thaliana immune receptor EFR in Medicago truncatula reduces infection by a root pathogenic bacterium, but not nitrogen-fixing rhizobial symbiosis. Plant Biotechnol J. doi: 10.1111/pbi.12999

Open Access

Sebastian Pfeilmeier and Jeoffrey George lead this work from the labs of Jacob Malone and Cyril Zipfel at the John Innes Centre and the Sainsbury lab, Norwich. In this study they have expressed the Arabidopsis PRR ELONGATION FACTOR-THERMO UNSTABLE RECEPTOR (EFR) immune reception in Medicago truncatula. They show that these transgenic plants remain able to form root nodules with the bacterial symbiont Sinorhizobium meliloti. However they are resistant to the bacterial pathogen Ralstonia solanacearum. This study shows the potential of cross-species approaches to develop broad-spectrum pathogen resistance. It will be interesting to learn more about future developments in this area.

Perraki A, DeFalco TA, Derbyshire P, Avila J, Séré D, Sklenar J, Qi X, Stransfeld L, Schwessinger B, Kadota Y, Macho AP, Jiang S, Couto D, Torii KU, Menke FLH, Zipfel C (2018) Phosphocode-dependent functional dichotomy of a common co-receptor in plant signalling. Nature doi: 10.1038/s41586-018-0471-x

This second study from the lab of Cyril Zipfel is led by Artemis Perraki and includes wide range of collaborators from across the globe. They have used phosphoproteomics and targeted mutagenesis to perform a detailed characterisation of the immune co-receptor BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1). This protein interacts with many leucine-rich repeat receptor kinases (LRR-RKs) yet they find that specific phosphosites discriminate between subsets of BAK1 functions that are linked to hormone or immune signaling. This study reveals new complexity in the regulation of this multi-facted protein and has broad importance regarding our understanding of how the phosphocode allows separation of the different signaling outputs.

Honkanen S, Thamm A, Arteaga-Vazquez MA, Dolan L (2018) Negative regulation of conserved RSL class I bHLH transcription factors evolved independently among land plants. Elife. doi: 10.7554/eLife.38529

Open Access

This study looks at the role of RSL class I basic helix-loop-helix transcription factors in the control of development in the lower plant Marchantia polymorpha. This work is led by Suvi Honkanen from Liam Dolan’s lab at the University of Oxford. In Arabidopsis RSL genes are negatively regulated by the GLABRA transcription factor yet in this study the authors identify a novel microRNA-based regulatory mechanism. Although RSL1 class I genes are evolutionarily conserved across land plants the miRNA regulatory module is only present in Marchantia, demonstrating that conserved genes can have divergent modes of regulation to control lineage-specific developmental requirements

Yu L, Lyczakowski JJ, Pereira CS, Kotake T, Yu X, Li A, Mogelsvang S, Skaf MS, Dupree P (2018) The patterned structure of galactoglucomannan suggests it may bind to cellulose in seed mucilage. Plant Physiol. doi: 10.1104/pp.18.00709

Open Access
This work is led by Li Yu from the lab of Paul Dupree at the University of Cambridge. They have investigated the detailed structure of mannose-based (mannan) polysaccharides within the Arabidopsis cell wall. These have previously been shown to be important in maintaining seed mucilage architecture, which has a glucose-mannose (glucomannan) backbone. The authors assess the contribution of the Cellulose Synthase-Like A2 (CSLA2) and Mannan α-Galactosyl Transferase 1 (MAGT1) enzymes in the construction and decoration of a galactoglucomannan backbone and provide data for molecular stimulations to predict as to how these might interact with cellulose microfibrils.

GARNet talks to Ari Sadanandom

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Published on: September 4, 2018

GARNet talked to Professor Ari Sadanandom from Durham University about a recent paper published in The Plant Cell entitled ‘SUMO Suppresses the Activity of the Jasmonic Acid Receptor CORONATINE INSENSITIVE 1

GARNet Research Roundup: August 22nd

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Published on: August 22, 2018

This week’s GARNet research roundup highlights outstanding science from across the UK. First are a group of three papers led by researchers in Cambridge and Bristol that investigate the role of either sugar or calcium signaling on control of the plant circadian clock. Secondly is work from Durham that provides an elegant link between SUMOylation and the jasmonate-responsive arm of the defence response. The biotrophic arm of defence signaling is the focus of the next paper from University of Nottingham that investigates the role of the N-end rule pathway in that response. Finally is a paper from the John Innes Centre that identifies a key determinant of planar cell polarity across the Arabidopsis leaf.

Frank A, Matiolli CC, Viana AJC, Hearn TJ, Kusakina J, Belbin FE, Wells Newman D, Yochikawa A, Cano-Ramirez DL, Chembath A, Cragg-Barber K, Haydon MJ, Hotta CT, Vincentz M, Webb AAR, Dodd AN (2018) Circadian Entrainment in Arabidopsis by the Sugar-Responsive Transcription Factor bZIP63. Current Biol. doi: 10.1016/j.cub.2018.05.092

Open Access

This research comes from the labs of Anthony Dodd (University of Bristol) and Alex Webb (University of Cambridge) and is led equally by Alexander Frank, Cleverson Matioli, Americo Viana, Timothy Hearn and Jelena Kusakina. They investigate how the Arabidopsis circadian clock is entrained to respond to changing metabolic rhythms, measured by assessing sugar signaling. The molecular factors that control changes in the circadian oscillator were previously unknown but they show that the transcription factor BASIC LEUCINE ZIPPER63 (bZIP63) is required to alter expression of the oscillator gene PSEUDO RESPONSE REGULATOR7 (PRR7). They also show that the SnRK1 sugar sensing kinase and TREHALOSE-6-PHOSPHATE SYNTHASE1 (TPS1) gene are required for sugar-mediated circadian adjustment. This study provides important information about additional layers of regulation controlling the relationship between the circadian clock and plant metabolism.

Martí Ruiz MC, Hubbard KE, Gardner MJ, Jung HJ, Aubry S, Hotta CT, Mohd-Noh NI, Robertson FC, Hearn TJ, Tsai YC, Dodd AN, Hannah M, Carré IA, Davies JM, Braam J, Webb AAR (2018) Circadian oscillations of cytosolic free calcium regulate the Arabidopsis circadian clock. Nat Plants. 2018 Aug 20. doi: 10.1038/s41477-018-0224-8

This second paper from the labs of Alex Webb and Anthony Dodd also features work from Isabelle Carre’s and Julia Davis’s lab in Warwick and Cambridge respectively. This work led by María Carmen Martí Ruiz, Katharine Hubbard and Michael J. Gardner looks at the how oscillations of cytoplasmic calcium influence the central circadian clock. They show that calcium influences the clock through the activity of the CALMODULIN-LIKE24 (CML24) gene and further genetic analysis links these activities through the action of the central clock gene TIMING OF CAB2 EXPRESSION1 (TOC1). This paper is also a clear lesson in persistence as it was first received by Nature Plants back in May 2016.

Ohara T, Hearn TJ, Webb AAR, Satake A. Gene regulatory network models in response to sugars in the plant circadian system. J Theor Biol. doi: 10.1016/j.jtbi.2018.08.020

The research includes members of Alex Webb’s group and develops a theoretical model to predict the response of the gene regulatory network that links the circadian clock to metabolic signals. This model predicts that the targets of sugar signaling could be both members of the PSEUDO-RESPONSE REGULATOR gene family as well as evening complex components. These findings are experimental confirmed in the paper by Frank et al in this edition of the GARNet response roundup.

Srivastava AK, Orosa B, Singh P, Cummins I, Walsh C, Zhang C, Grant M, Roberts MR, Anand GS, Fitches E, Sadanandom A (2018) SUMO Suppresses the Activity of the Jasmonic Acid Receptor CORONATINE INSENSITIVE 1. Plant Cell. doi: 10.1105/tpc.18.00036

Open Access

Lead author on this paper from the labs of Elaine Fitches and Ari Sadanandom at the Unversity of Durham is Anjil Kumar Srivastava and includes co-authors from Lancaster University. In the study they reveal a feedback loop between the jasmonic acid receptor CORONATINE INSENSITIVE 1 (COI1) and its targets for degradation; the JASMONATE ZIM (JAZ) domain-containing repressor proteins. The authors show that SUMOylated JAZ proteins inhibit the COI1-dependent degradation of non-SUMOylated JAZ proteins. In addition they identify a SUMO-responsive element within the COI1 protein and that necrotrophic bacteria specifically target SUMO protease in order to modulate JA-responsive defense responses.

Vicente J, Mendiondo GM, Pauwels J, Pastor V, Izquierdo Y, Naumann C, Movahedi M, Rooney D, Gibbs DJ, Smart K, Bachmair A, Gray JE, Dissmeyer N, Castresana C, Ray RV, Gevaert K, Holdsworth MJ (2018) Distinct branches of the N-end rule pathway modulate the plant immune response. New Phytol. doi: 10.1111/nph.15387

Open Access
Jorge Vicente leads this work from the lab of Mike Holdsworth in Nottingham that includes collaborators from Belgium, Spain, Germany and Austria. They investigate the role of the N-end rule degradation pathway in the plant immune response. Indeed they show that portions of this response mediated by the E3 ligase PROTEOLYSIS (PRT)6 are important for expression of a specific set of defense-related genes and basal resistance to a biotropic pathogen. They also show this response is also important in the monocot barley where plants with reduced expression of HvPRT6 have enhanced resistance to different pathogens.

Mansfield C, Newman JL, Olsson TSG, Hartley M, Chan J, Coen E (2018) Ectopic BASL Reveals Tissue Cell Polarity throughout Leaf Development in Arabidopsis thaliana. Curr Biol. doi: 10.1016/j.cub.2018.06.019

Open Access
Catherine Mansfield leads this study from the lab of Enrico Coen at the John Innes Centre that investigates the factors that control cell polarity during leaf development. They show that BASL (BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE) is essential for establishment an organ-wide polarity field across the Arabidopsis leaf. They show this polarity field is aligned with the proximodistal axis of the leaf (base to tip) and is independent of stomatal patterning. Like in animals this demonstrates that planar plant organs have a tissue-wide cell polarity field that might be critical for guiding growth and differentiation.

GARNet Research Roundup: August 10th 2018

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Published on: August 10, 2018

There are three papers in this week’s GARNet research roundup. The first paper is led by Jill Harrison’s lab in Bristol and she also provides an audio description of this work that has characterised a role for CLAVATA genes in the transition from 2D to 3D plant growth. The second paper from Ian Graham’s lab in York introduces the role of the MOTHER-OF-FT-AND-TFL1 gene during seed germination whilst the final paper includes co-authors from SLCU and Nottingham and has identified the RALF34 protein as a novel ligand that influences cell wall growth.

Whitewoods CD, Cammarata J, Nemec Venza Z, Sang S, Crook AD, Aoyama T, Wang XY, Waller M, Kamisugi Y, Cuming AC, Szövényi P, Nimchuk ZL, Roeder AHK, Scanlon MJ, Harrison CJ (2018) CLAVATA Was a Genetic Novelty for the Morphological Innovation of 3D Growth in Land Plants. Curr Biol. doi: 10.1016/j.cub.2018.05.068

Open Access

Chris Whitewoods and Joe Cammarata are co-first authors in this UK-US-Japan collaboration that is led by GARNet committee member Jill Harrison from the University of Bristol. They have investigated the expression and function of CLAVATA genes during moss development with particular focus on the transition from 2D to 3D growth, showing that these genes are essential for gametophyte development. By showing the presence or absence of CLAVATA genes in different lower plant species they demonstrate that they are important for the transition between different modes of growth.

Jill talks about this work on the GARNet YouTube channel and podcast.

Vaistij FE, Barros-Galvão T, Cole AF, Gilday AD, He Z, Li Y, Harvey D, Larson TR, Graham IA (2018) MOTHER-OF-FT-AND-TFL1 represses seed germination under far-red light by modulating phytohormone responses in Arabidopsis thaliana. Proc Natl Acad Sci U S A. doi: 10.1073/pnas.1806460115

Open Access

Fabian Vaistij is the first author on this paper from the lab of Ian Graham at the University of York that investigates the role of the MOTHER-OF-FT-AND-TFL1 (MFT) in the control of seed germination in Arabidopsis. They show that MFT is upregulated by far red light via the previously characterised PIF1/SOM/ABI5/DELLA pathway whilst repressed by red light through the action of the SPATULA (SPT) transcription factor. The activity of MFT alters levels of ABA and GA that ultimately delay germination in conditions with higher levels of FR light.

Gonneau M, Desprez T, Martin M, Doblas VG, Bacete L, Miart F, Sormani R, Hématy K, Renou J, Landrein B, Murphy E, Van De Cotte B, Vernhettes S, De Smet I, Höfte H (2018) Receptor Kinase THESEUS1 Is a Rapid Alkalinization Factor 34 Receptor in Arabidopsis. Curr Biol. doi: 10.1016/j.cub.2018.05.075
This French-led study that has Martine Gonneau and Thierry Desprez as co-lead authors includes Benoit Landrien (SLCU, Cambridge) and Evan Murphy (University of Nottingham) as co-authors. This research adds further detail to a signaling network that coordinates cell wall growth following different stimuli. They show that the rapid peptide alkalinization factor 34 (RALF34) is the ligand for the previously characterized THESEUS1 receptor kinase and that the activity of this signaling module is dependent on FERONIA, which is another RALF receptor.

Jill Harrison talks to GARNet

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Published on: August 6, 2018

Jill Harrison discusses a recent Current Biology paper entitled ‘CLAVATA Was a Genetic Novelty for the Morphological Innovation of 3D Growth in Land Plants

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